|
KMID : 1149220190270010017
|
|
Korean Journal of Oriental Medical Prescription
2019 Volume.27 No. 1 p.17 ~ p.29
|
|
|
Sipyukmiryuki-eum Exhibits Anti-inflammatory and Anti-oxidative Effect viaActivation of Nrf2/HO-1 Signaling in Lipopolysaccharide-stimulated RAW264.7 Macrophages
|
|
Kwon Da-Hye
Hwang Young-Hoon
Kim Min-Young
Ji Seon-Yeong
Hong Su-Hyun
Park Cheol
Hwang Hye-Jin
Choi Yung-Hyun
|
|
|
Abstract
|
|
|
|
Inflammatory and oxidative stimuli play a critical role not only in the process of transforming normal cells into cancer cells, but also in the proliferation process of cancer cells. Sipyukmiryukieum (SYMRKU), a traditional Korean herb-combined remedy, is composed of 16 kinds of herbal medicines, which were recorded for “Ongjeo” treatment in “Dongeuibogam’. In this study, we investigated the inhibitory effect of SYMRKU against inflammatory and oxidative responses in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. Our results showed that SYMRKU significantly inhibited LPS-induced secretion of pro-inflammatory mediators including nitric oxide (NO) and prostaglandin E2 without showing any significant cytotoxicity. Consistent with these results, SYMRKU down-regulated LPS-induced expression of their regulatory enzymes such as inducible NO synthase and cyclooxygenase-2. SYMRKU also inhibited LPS-induced production and expression of pro-inflammatory cytokines such as tumor necrosis factor-α, interleukin (IL)-1β and IL-6. In addition, SYMRKU significantly reduced the production of reactive oxygen species by LPS and showed a strong, which was associated with induction of nuclear factor erythroid 2-related factor 2 and heme oxygenase-1 expression. Although further studies are needed to fully understand the anti-inflammatory effects associated with the antioxidant capacity of SYMRKU, the findings of the current study suggest that SYMRKU may have potential benefits by inhibiting the onset and/or treatment of inflammatory and/or oxidative diseases.
|
|
|
KEYWORD
|
|
|
Sipyukmiryukieum, RAW 264.7 macrophages, anti-inflammation, antioxidant, Nrf2/HO-1
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|